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Description:Cells were grown in monolayer culture, harvested by trypsinization, and implanted subcutaneously into the right flank of 6- to 8-week-old female Balb/C (CT26) or C57BL/6 (MCA), or 4- to 6-week-old athymic female nude mice (Harlan, Indianapolis, IN). For the CT26 tumor model, 5 × cells were.